東北大学多元物質科学研究所
細胞機能分子化学研究分野 水上研究室

2023

• Arylazopyrazole-Based Photoswitchable Inhibitors Selective for Escherichia coli Dihydrofolate Reductase^NEW
  H. S. Sarkar, T. Mashita, T. Kowada, S. Hamaguchi, T. Sato, K. Kasahara, N. Matubayasi, T. Matsui, and S. Mizukami
  ACS Chem. Biol., (2023) XXX, XXX–XXX.
  

2022

• Clip to Click: Controlling Inverse Electron-Demand Diels–Alder Reactions with Macrocyclic Tetrazines^NEW
  I. Novianti, T. Kowada, and S. Mizukami
  Org. Lett., (2022) 24, 3223–3226.
  

• Organelle-Level Labile Zn²⁺ Mapping Based on Targetable Fluorescent Sensors^NEW
  R. Liu, T. Kowada, Y. Du, Y. Amagai, T. Matsui, K. Inaba, and S. Mizukami
  ACS Sens., (2022) 7, 748–757.
  

• Flexible Target Recognition of the Intrinsically Disordered DNA-Binding Domain of CytR Monitored by Single-Molecule Fluorescence Spectroscopy^NEW
  Shrutarshi Mitra, Hiroyuki Oikawa, Divya Rajendran, Toshiyuki Kowada, Shin Mizukami, Athi N. Naganathan, and Satoshi Takahashi
  J. Phys. Chem. B, (2022) 126, 6136–6147.
  

• Autocitrullination and Changes in the Activity of Peptidylarginine Deiminase 3 Induced by High Ca²⁺ Concentrations
  M. Sawata, H. Shima, K. Murayama, T. Matsui, K. Igarashi, K. Funabashi, K. Ite, K. Kizawa, H. Takahara, and M. Unno
  ACS Omega, (2022) 7, 28378–28387.
  

• 細胞機能を探索するための光応答性プローブの開発^NEW
  水上 進
  YAKUGAKU ZASSHI, (2022) 142, 503–511.

• ヘム分解酵素の多様な分子機構
  松井敏高、齋藤正男
  ヘムタンパク質の科学（(株) エヌ・ティー・エス）, 第1編4章2節, 159-167 (2022).

2021

• Fluorescent Probes for the Quantification of Labile Metal Ions in Living Cells
  T. Kowada and S. Mizukami
  J. Synth. Org. Chem. Jpn., (2021) 79 , 1020-1032.
• Optical manipulation of subcellular protein translocation using a photoactivatable covalent labeling system
  T. Kowada, K. Arai, A. Yoshimura, T. Matsui, K. Kikuchi, S. Mizukami
  Angew. Chem. Int. Ed.,  (2021)  60, 11378-11383.
  Introduced in Chem-Station Spotlight Research.
  
• Protocol for synthesis and use of a turn-on fluorescent probe for quantifying labile Zn²⁺ in the Golgi apparatus in live cell
  T. Kowada, T. Watanabe, R. Liu, S. Mizukami
  STAR Protoc., (2021) 2 , 100395.
• Long-Term Mg²⁺ Imaging in Live Cells with a Targetable Fluorescent Probe
  P. R. Sahoo, T. Kowada, S. Mizukami
  Methods in Molecular Biology (Humana, New York, NY), vol. 2274 Live Cell Imaging pp.237-243.
• Functional small-molecule probes for fluorescence imaging of osteoclastsin vivo
  T. Kowada
  Bioimages, (2021) 29 , 1–10.

2020

• Quantitative imaging of labile Zn²⁺ in the Golgi apparatus using a localizable small-molecule fluorescent probe
  T. Kowada, T. Watanabe, Y. Amagai, R. Liu, M. Yamada, H. Takahashi, T. Matsui, K. Inaba , S. Mizukami
  Cell Chem. Biol.,  (2020) 27 , 1521-1531.

• Single-cell dynamics of pannexin-1-facilitated programmed ATP loss during apoptosis
  H. Imamura, S. Sakamoto, T. Yoshida, Y. Matsui, S. Penuela, DW Laird, S. Mizukami, K. Kikuchi, A. Kakizuka
  eLife ,  ( 2020)  9 , e61960.
   

• Unique Electronic Structures of the Highly Ruffled Hemes in Heme-Degrading Enzymes of Staphylococcus aureus, IsdG and IsdI, by Resonance Raman and Electron Paramagnetic Resonance Spectroscopies
  S. Takahashi, S. Nambu, T. Matsui, H. Fujii, H. Ishikawa, Y. Mizutani, K. Tsumoto, M. Ikeda-Saito
  Biochemistry, (2020) 59, 3918-3928.

• Structure determination of the human TRPV1 ankyrin-repeat domain under nonreducing conditions
  M. Tanaka, K. Hayasaka, N. Ogawa, T. Kurokawa, K. Kitanishi, K. Ite, T. Matsui, Y. Mori, M. Unno
  Acta Cryst., (2020) F76, 130-137.

• 光による生体分子機能の制御
  水上進
  ［CSJカレントレビュー36］生体分子反応を制御する　化学的手法による機構と反応場の解明（化学同人出版）, 第Ⅱ部2章, p.51-56

2019

• Improvement in Photostability of Fluorescein by Lanthanide Ions based on Energy Transfer-Based Triplet State Quenching
  T. Imoto, M. Muramatsu, H. Miyasaka, S. Mizukami, K. Kikuchi
  Chem. Lett., (2019) 48, 1181-1184.

• Biophysical characterization of heme binding to the intrinsically disordered region of Bach1
  K. Segawa, M. Watanabe-Matsui, K. Tsuda , T. Matsui, M. Shirouzu , K. Igarashi , K. Murayama
  Eur. Biophys. J., (2019) 48, 361-369.

• Functional Heme Binding to the Intrinsically Disordered C-Terminal Region of Bach1, a Transcriptional Repressor
  K. Segawa, M. Watanabe-Matsui, T. Matsui, K. Igarashi, K. Murayama
  Tohoku J. Exp. Med., (2019) 247, 153-159.

• Light-wavelength-based Quantitative Control of Dihydrofolate Reductase Activity Using Photochromic Isostere of Inhibitor
  T. Mashita, T. Kowada, H. Takahashi, T. Matsui, S. Mizukami
  ChemBioChem, (2019) 20, 1382-1386.

2018

• Perfluorocarbon-based ¹⁹F MRI Nanoprobes for In Vivo Multicolor Imaging
  K. Akazawa, F. Sugihara, T. Nakamura, H. Matsushita, H. Mukai, R. Akimoto, M. Minoshima, S. Mizukami, K. Kikuchi
  Angew. Chem., Int. Ed., (2018) 57, 16742-16747.

• Hydrogen sulfide bypasses the rate-limiting oxygen activation of heme oxygenase
  T. Matsui, R. Sugiyama, K. Sakanashi, Y. Tamura, M. Iida, Y. Nambu, T. Higuchi, M. Suematsu, M. Ikeda-Saito
  J. Biol. Chem., (2018) 293, 16931-16939.
  

• MRIなどへの機能性含フッ素プローブ応用
  水上進
  有機フッ素化合物の最新動向（シーエムシー出版）, 第2章3節, pp.151–160 (2018). 

• Sensing Caspase-1 Activity Using Activatable ¹⁹F MRI Nanoprobes with Improved Turn-on Kinetics
  K. Akazawa, F. Sugihara, M. Minoshima, S. Mizukami, K. Kikuchi
  Chem. Commun., (2018) 54, 11785-11788 

• Highly Sensitive Detection of Caspase-3/7 Activity in Living Mice Using Enzyme-Responsive ¹⁹F MRI Nanoprobes
  K. Akazawa, F. Sugihara, T. Nakamura, S. Mizukami, K. Kikuchi
  Bioconjugate Chem., (2018) 29, 1720–1728. 

• Ratiometric Imaging of Intracellular Mg²⁺ Dynamics Using a Red Fluorescent Turn-off Probe and a Green Fluorescent Turn-on Probe
  Y. Matsui, S. Mizukami, K. Kikuchi
  Chem. Lett., (2018) 47, 23–26. 

• 蛋白質ラベル化技術atiometric Imaging of 実験医学増刊「イメージングの選び方・使い方100+」p.147-149（羊土社, 1018年）
  小和田俊行, 水上進
  

2017

• Intracellular protein-labeling probes for multicolor single-molecule imaging of immune receptor-adaptor molecular dynamics
  R. Sato, J. Kozuka, M. Ueda, R. Mishima, Y. Kumagai, A. Yoshimura, M. Minoshima, S. Mizukami, K. Kikuchi
  J. Am. Chem. Soc., (2017) 139, 17397–17404. 

• Visualization of Long-term Mg²⁺ Dynamics in Apoptotic Cells with a Novel Targetable Fluorescent Probe
  Y. Matsui, Y. Funato, H. Imamura, H. Miki, S. Mizukami, K. Kikuchi
  Chem. Sci., (2017) 8, 8255-8264. 

• Highly selective tridentate fluorescent probes for visualizing intracellular Mg²⁺ dynamics without interference from Ca²⁺ fluctuation
  Y. Matsui, K. K. Sadhu, S. Mizukami, K. Kikuchi
  Chem. Commun., (2017) 53, 10644-10647 

• Enzyme-Triggered Compound Release using Functionalized Antimicrobial Peptide Derivatives
  S. Mizukami, M. Kashibe, K. Matsmoto, Y. Hori, K. Kikuchi
  Chem. Sci., (2017) 8, 3047-3053 

• Targetable Fluorescent Sensors for Advanced Cell Function Analysis
  S. Mizukami
  J. Photochem. Photobiol. C (2017) 30, 24-35 

• Reaction intermediates in the heme degradation reaction by HutZ from Vibrio cholera 
  T. Uchida, S. Sekine, N. Dojun, A. Lewis-Ballester, I. Ishigami, T. Matsui, S. Yeh, K. Ishimori
  Dalton Trans., (2017) 46, 8104-8109

2016

• Real-time Intravital Imaging of pH Variation Associated with Osteoclast Activity
  H. Maeda, T. Kowada, J. Kikuta, M. Furuya, M. Shirazaki, S. Mizukami, M. Ishii, K. Kikuchi
  Nat. Chem. Biol., (2016) 12, 579-585 [ISI] [プレスリリース]

• Unique coupling of mono- and dioxygenase chemistries in a single active site promotes heme degradation
  T. Matsui, S. Nambu, C. W. Goulding, S. Takahashi, H. Fujii, M. Ikeda-Saito
  Proc. Natl. Acad. Sci. (2016) 113, 3779-84 [ISI] [プレスリリース]

• Artificial Hydrogenases based on Cobaloximes and Heme Oxygenase
  M. Bacchi, E. Veinberg, M. J. Field, J. Niklas, T. Matsui, D. M Tiede, O. G. Poluektov, M. Ikeda-Saito, M. Fontecave V. Artero
  ChemPlusChem (2016) 81, 1083-1089

• Charge-state-distribution analysis of Bach2 intrinsically disordered heme binding
  T. Suenaga, M. Watanabe-Matsui, H. Shima, T. Matsui, M. Ikeda-Saito, K. Igarashi, K. Murayama
  J. Biochem. (2016)  160 , 291-298.
  

Before 2015

　・Professor Mizukami

　・Associate Professor Matsui

　・Assistant Professor Kowada